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MASTOPARAN INHIBITS PHOSPHOINOSITIDE HYDROLYSIS VIA PERTUSSIS TOXIN-INTENSIVE G-PROTEIN IN HUMAN ASTROCYTOMA-CELLS

被引：54
作者：
NAKAHATA, N
ABE, MT
MATSUOKA, I
NAKANISHI, H
机构：
[1] Department of Pharmacology, Fukushima Medical College, 960-12, Hikarigaoka 1, Fukuishima
来源：
FEBS LETTERS | 1990年 / 260卷 / 01期
关键词：
GTP binding protein; Inositol phosphate; Mastoparan; Pertussis toxin; Phospholipase C;
D O I：
10.1016/0014-5793(90)80074-S
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Mastoparan inhibited [3H]inositol phosphate accumulation induced by carbachol as well as cyclic AMP accumulation induced by isoproterenol in 1321N1 human astrocytoma cells. Mastoparan inhibited GTPγS-induced, but not Ca2+-induced, [3H]inositol phosphate accumulation in membrane preparations with an IC50 of approximately 10 μM. The inhibitory effect of mastoparan on carbachol-induced [3pH]inositol phosphate accumulation was resistant to pertussis toxin (IAP) treatment in intact cells. These results suggest that mastoparan inhibits phospholipase C in human astrocytoma cells via a GTP binding protein, which is not a substrate for IAP. © 1990.
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页码：91 / 94
页数：4
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