PREPARATIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF INDIVIDUAL AMINO-ACIDS DERIVED FROM FOSSIL BONE-COLLAGEN

被引:39
作者
VANKLINKEN, GJ [1 ]
MOOK, WG [1 ]
机构
[1] STATE UNIV GRONINGEN, CENT ISOTOPENONDERZOEK, 9718 CM GRONINGEN, NETHERLANDS
关键词
D O I
10.1017/S0033822200040157
中图分类号
P3 [地球物理学]; P59 [地球化学];
学科分类号
0708 ; 070902 ;
摘要
Extracting a series of amino acids from fossil "collagen' makes it possible to compare chemically well-characterized molecules from one bone sample, both chemically and by isotopic analysis. Thus, the integrity of collagen samples and possible contamination can be checked. The routine high-performance liquid chromatographic extraction of five amino acids: glycine, hydroxyproline, glutamate, threonine and alanine is described. Humates are eliminated during the extraction, which can be monitored continuously. -Authors
引用
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页码:155 / 164
页数:10
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