MICRO-AUTO-RADIOGRAPHIC STUDY OF THE ACTIVITY OF ATTACHED AND FREE-LIVING BACTERIA

被引：38

作者：

FLETCHER, M

机构：

[1] Department of Environmental Sciences, University of Warwick, Coventry

来源：

ARCHIVES OF MICROBIOLOGY | 1979年 / 122卷 / 03期

关键词：

Activity of; Attachment substrata; Bacterial attachment; Marine pseudomonad; Microautoradiography;

D O I：

10.1007/BF00411290

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Microautoradiography, combined with epifluorescent microscopy, was used to evaluate the uptake of tritiated amino acids by a marine Pseudomonas sp. A comparison was made between the activity of bacteria free-living in the medium and bacteria which were attached to glass, polyethylene or polystyrene substrata. The proportion of active bacteria was lower for free-living cells (53-82%) and those attached to polystyrene (53-76%) than for those attached to glass (77-99%) or polyethylene (73-96%). For bacteria attached to glass, assimilated labelled substrate was retained within the cell over 3 h, whereas with polyethylene, labelled material was released from the cells and adsorbed on the surrounding substratum. Hence the physiological activity of attached bacteria depended on the chemical composition of the substratum. © 1979 Springer-Verlag.

引用

页码：271 / 274

页数：4

共 20 条

[1]

Andrade J D, 1973, Med Instrum, V7, P110

[2]

Corpe W.A., 1970, ATTACHMENT MARINE BA, V73

[3] ELECTRON-MICROSCOPIC DEMONSTRATION OF AN ACIDIC POLYSACCHARIDE INVOLVED IN ADHESION OF A MARINE BACTERIUM TO SOLID SURFACES [J].