GENE PROBES FOR THE DETECTION OF 6-DEOXYHEXOSE METABOLISM IN SECONDARY METABOLITE-PRODUCING STREPTOMYCETES

被引:39
作者
STOCKMANN, M
PIEPERSBERG, W
机构
关键词
6-DEOXYHEXOSE METABOLISM; DNA PROBE; STREPTOMYCETE;
D O I
10.1111/j.1574-6968.1992.tb05149.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
DNA probes were designed from the streptomycin production genes strDELM of Streptomyces griseus involved in the biosynthesis of the 6-deoxyhexose (6DOH) dihydrostreptose which could detect the genomic fragments coding for 6DOH formation in other actinomycetes strains. In about 70% of the 43 strains tested at least one signal could be detected with strD-, strE- or strLM-specific probes. Evidence is presented that the hybridizing genes are mostly clustered and probably engaged in the formation of secondary metabolites. Because of the wide-spread use of 6DOH constituents in natural products these probes should allow to detect a vast array of different secondary metabolic gene clusters in actinomycetes.
引用
收藏
页码:185 / 190
页数:6
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