PHYSICAL ANALYSIS OF THE TERMINAL-240 KB OF DNA FROM HUMAN CHROMOSOME-7Q

被引：24

作者：

RIETHMAN, HC ^{[1
]}

SPAIS, C ^{[1
]}

BUCKINGHAM, J ^{[1
]}

GRADY, D ^{[1
]}

MOYZIS, RK ^{[1
]}

机构：

[1] LOS ALAMOS NATL LAB,CTR HUMAN GENOME STUDIES,LOS ALAMOS,NM 87544

来源：

GENOMICS | 1993年 / 17卷 / 01期

关键词：

D O I：

10.1006/geno.1993.1278

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

DNA from a 240-kb human telomeric yeast artificial chromosome (HTY) clone was analyzed using physical mapping methods. Cosmid subclones of the YAC were fingerprinted using restriction enzyme digestion and human repeat sequence hybridization and then assembled into two contigs that together span 93% of the human insert. Data from restriction mapping and Bal31 exonuclease experiments indicate that, except for the truncation of distal genomic (T2AG3)(n) sequences, the molecular clone HTY 146 contains a contiguous, 230-kb telomere-terminal fragment from 7qter. Markers derived from this clone will allow telomeric closure of the physical and genetic linkage maps of human chromosome 7q. © 1993 Academic Press, Inc.

引用

页码：25 / 32

页数：8

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