BANDING OF G-TYPE OR R-TYPE IN PROPHASE PROMETAPHASE IN THE M-CHROMOSOME OF VICIA-FABA L

The differential bandings of the M-chromosome of Vicia faba, produced in the period from prophase to metaphase of the mitotic cycle, has been studied by means of pretreatment with low positive temperatures. Staining was performed with the fluorochromes Hoechst 33258 and olivomycin and with acetocarmine. The result is a stable picture of differential banding of euchromatic regions similar to the G- or R-type bandings of animal chromosomes. About 15/17 bands, depending on staining, have been identified and charted. There is an inversely proportional relation between the degree of condensation of the bands during prophase and the fluorescence intensity of the dye Hoechst 33258.