MONOCLONAL-ANTIBODIES TARGETED AGAINST THE C-TERMINAL DOMAIN OF DYSTROPHIN OR UTROPHIN

被引：36

作者：

FABBRIZIO, E ^{[1
]}

LEGER, J ^{[1
]}

ANOAL, M ^{[1
]}

LEGER, JJ ^{[1
]}

MORNET, D ^{[1
]}

机构：

[1] FAC PHARM MONTPELLIER,INSERM,U300,UNITE PHYSIOPATHOL CELLULAIRE & MOLEC,BATIMENT K,F-34060 MONTPELLIER,FRANCE

来源：

FEBS LETTERS | 1993年 / 322卷 / 01期

关键词：

DYSTROPHIN; UTROPHIN; MONOCLONAL ANTIBODY; EPITOPE MAPPING; RECOMBINANT PROTEIN;

D O I：

10.1016/0014-5793(93)81100-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure-function relationships of dystrophin, a protein which is absent or defective in patients with Duchenne or Becker muscular dystrophies, and utrophin can only be compared if specific antibodies are produced. We expressed C-terminal parts of dystrophin and utrophin in expression vectors. Mice were immunized with recombinant proteins and 26 monoclonal antibodies were produced and analyzed. Their respective epitopes were determined using other overlapping recombinant products. We observed antibody specificity towards 400 kDa dystrophin and/or utrophin protein bands, either by Western blot analysis or immunodetection in human skeletal (quadriceps) and smooth (uterus) muscles. These antibodies have been used to compare the relative abundance of both dystrophin and utrophin relative to the structures analyzed.

引用

页码：10 / 14

页数：5

共 26 条

[1] A NOVEL PRODUCT OF THE DUCHENNE MUSCULAR-DYSTROPHY GENE WHICH GREATLY DIFFERS FROM THE KNOWN ISOFORMS IN ITS STRUCTURE AND TISSUE DISTRIBUTION [J].