EFFICIENT GENE-TRANSFER IN SYNECHOCOCCUS SP STRAINS PCC-7942 AND PCC-6301 BY INTERSPECIES CONJUGATION AND CHROMOSOMAL RECOMBINATION

被引：55

作者：

TSINOREMAS, NF ^{[1
]}

KUTACH, AK ^{[1
]}

STRAYER, CA ^{[1
]}

GOLDEN, SS ^{[1
]}

机构：

[1] TEXAS A&M UNIV, DEPT BIOL, COLLEGE STN, TX 77843 USA

来源：

JOURNAL OF BACTERIOLOGY | 1994年 / 176卷 / 21期

关键词：

D O I：

10.1128/JB.176.21.6764-6768.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a versatile, efficient genetic transfer method for Synechococcus sp. strains PCC 7942 and PCC 6301 that exceeds natural transformation efficiencies by orders of magnitude. As a test case, we complemented a histidine auxotroph and identified a hisS homolog of PCC 7942 as the complementing gene.

引用

页码：6764 / 6768

页数：5

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