NEW IMMUNOASSAY FOR THE MAPPING OF NEUROFIBRILLARY DEGENERATION IN ALZHEIMERS-DISEASE USING 2 MONOCLONAL-ANTIBODIES AGAINST HUMAN PAIRED HELICAL FILAMENT TAU PROTEINS

被引：11

作者：

CONDAMINES, O

BUEESCHERRER, V

BOISSIER, L

WATTEZ, A

DELACOURTE, A

PAU, B

MOURTONGILLES, C

机构：

[1] FAC PHARM MONTPELLIER,CNRS,UMR 9921,F-34060 MONTPELLIER,FRANCE

[2] INSERM,U422,F-59045 LILLE,FRANCE

来源：

NEUROSCIENCE LETTERS | 1995年 / 192卷 / 02期

关键词：

ANTIGEN-CAPTURE ELISA; PHF-TAU PROTEINS; ALZHEIMERS DISEASE; MONOCLONAL ANTIBODY;

D O I：

10.1016/0304-3940(95)11617-6

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Monoclonal antibodies against human paired helical filament tau (PHF-tau) proteins were produced. Two of these antibodies, AD1 and AD2, were shown by immunoblot to be directed against distinct hyperphosphorylated epitopes of the PHF-tau proteins. Using AD1 and AD2, an antigen-capture ELISA specific for PHF-tau proteins was developed and used to map the neurofibrillary degeneration of several Broadmann areas from an Alzheimer's disease patient. The results confirm that the neurofibrillary degeneration predominates in parietal and temporal lobes.

引用

页码：81 / 84

页数：4

共 21 条

[1]

Delacourte, Defossez, Alzheimer's disease: tau proteins, the promoting factors of microtubule assembly, are major components of paired helical filaments, J. Neurol. Sci., 76, pp. 173-186, (1986)

[2]

Delacourte, Flament, Dibe, Hublau, Sablonniere, Hemon, Scherrer, Defossez, Pathological proteins tau 64 and 69 are specifically expressed in the somatodendritic domain of the degenerating cortical neurons during Alzheimer's disease. Demonstration with a panel of antibodies against tau proteins, Acta Neuropathol., 80, pp. 111-117, (1990)

[3]

Drechsel, Hyman, Cobb, Kirschner, Modulation of the dynamic instability of tubulin assembly by the microtubule-associated protein tau, Mol. Cell. Biol., 3, pp. 1141-1154, (1992)

[4]

Flament, Delacourte, Abnormal tau species are produced during Alzheimer's disease neurodegenerating process, FEBS Lett, 247, pp. 213-216, (1989)

[5]

Ghanbari, Kosuk, Miller, Reising, A sandwich enzyme immunoassay for detecting and measuring Alzheimer's disease associated proteins (ADAP) in human brain tissue, J. Clin. Lab. Anal., 4, pp. 189-192, (1990)

[6]

Goedert, Spillantini, Jakes, Rutherford, Crowther, Multiple isoforms of human microtubule-associated protein tau: sequences and localization in eurofibrillary tangles of Alzheimer's disease, Neuron, 3, pp. 519-526, (1989)

[7]

Goedert, Jakes, Crowther, Six, Lubke, Vandermeeren, Cras, Trojanowski, Lee, The abnormal phosphorylation of tau proteins at Ser 202 in Alzheimer's disease recapitulates phosphorylation during development, Proceedings of the National Academy of Sciences, 90, pp. 5066-5070, (1990)

[8]

Greenberg, Davies, A preparation of Alzheimer paired helical filaments that displays distinct tau proteins by polyacrylamide gel electrophoresis, Proc. Natl. Acad. Sci. USA., 87, pp. 5827-5831, (1990)

[9]

Grundke-Iqbal, Tung, Quilan, Wisniewski, Binder, Abnormal phosphorylation of the microtubule associated protein (tau) in Alzheimer's disease cytoskeletal pathology, Proceedings of the National Academy of Sciences, 83, pp. 4913-4917, (1986)

[10]

Holzer, Holzpfel, Zedlick, Bruckner, Arendt, Abnormally phosphorylated tau protein in Alzheimer's disease: heterogeneity of individual regional distribution and relationship to clinical severity, Neuroscience, 63, pp. 499-516, (1994)

← 1 2 3 →