EXTRACTION AND ANALYSIS OF AMINO ACIDS FROM APPLE TREE MATERIAL

被引：15

作者：

HILLCOTTINGHAM, DG

COOPER, DR

机构：

[1] University of Bristol, Department of Agriculture and Horticulture, Research Station, Bristol, BS18 9AF, Long Ashton

来源：

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | 1969年 / 20卷 / 11期

关键词：

D O I：

10.1002/jsfa.2740201108

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Two extractants are suggested for use with apple tree material: ethanol/water/N‐HCI, 75 : 25 :2 by vol., for all tissues except leaves; and ethanol‐chloroform‐water, 12 : 5 : 3 by vol., for leaves. The ability of these solutions to extract the nitrogenous constituents of apple tree tissues has been compared with that of other extractants. Some improvements are suggested for the analysis of amides by hydrolysis and of amino acids by thin‐layer electrophoresis/thin‐layer chromatography. Details of the recommended methods for freeze‐drying and milling tree material and for the extraction and quantitative analysis of amino acids are also given. Copyright © 1969 John Wiley & Sons, Ltd

引用

页码：662 / +

页数：1

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