SPECIES-SPECIFIC DETECTION OF LISTERIA-MONOCYTOGENES BY DNA AMPLIFICATION

被引:91
作者
DENEER, HG [1 ]
BOYCHUK, I [1 ]
机构
[1] ROYAL UNIV HOSP,DEPT CLIN MICROBIOL,SASKATOON S7N 0W0,SASKATCHEWAN,CANADA
关键词
D O I
10.1128/AEM.57.2.606-609.1991
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The polymerase chain reaction was used to detect and specifically identify Listeria monocytogenes. A 174-bp region of the listeriolysin O gene was shown to be specifically amplified in L. monocytogenes but not in other species of Listeria or in a number of other gram-positive and gram-negative organisms. Less than 50 organisms could routinely be detected by a procedure involving two rounds of 35 amplification cycles each and without the need for subsequent hybridization with labeled probes.
引用
收藏
页码:606 / 609
页数:4
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