TRANSIENT ELECTRIC BIREFRINGENCE OF T7-VIRAL DNA

被引:39
作者
RAU, DC
BLOOMFIELD, VA
机构
[1] Department of Biochemistry, University of Minnesota, St. Paul, Minnesota
关键词
D O I
10.1002/bip.1979.360181110
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The electric birefringence properties of intact bacteriophage T7 DNA are investigated as a function of field strength and ionic environment. At low field strengths, E < 40 V/cm, Kerr behavior is observed. The birefringence decay kinetics are consistent with a Gaussian chain viscoelastic treatment for an induced dipole model of orientation. The observed relaxation time for the longest mode is 37 msec, in 0.5 mM Na+ at 15°C, and is in agreement with flow experiments. In order to account for the observed differences in rise and decay kinetics, a field‐induced, anharmonic restoring force is postulated. The magnitude of the apparent induced dipole moment is found to be inversely proportional to the Debye‐Hückel shielding parameter κ for a series of Na+ concentrations. The magnitude of the induced dipole moment is found to be very sensitive to the addition of Mg2+. When analyzed in terms of the counterion condensation theory of Manning, it appears that the induced dipole moment is proportional to the square of the reduced effective DNA charge density. Copyright © 1979 John Wiley & Sons, Inc.
引用
收藏
页码:2783 / 2805
页数:23
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