PURIFICATION AND EVALUATION OF THE INTEGRAL MEMBRANE-PROTEIN H11 AS A PROTECTIVE ANTIGEN AGAINST HAEMONCHUS-CONTORTUS

被引：110

作者：

SMITH, TS ^{[1
]}

MUNN, EA ^{[1
]}

GRAHAM, M ^{[1
]}

TAVERNOR, AS ^{[1
]}

GREENWOOD, CA ^{[1
]}

机构：

[1] AFRC, INST ANIM PHYSIOL & GENET RES, DEPT IMMUNOL, CAMBRIDGE RES STN, CAMBRIDGE CB2 4AT, ENGLAND

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1993年 / 23卷 / 02期

关键词：

HAEMONCHUS-CONTORTUS; MEMBRANE PROTEINS; VACCINATION;

D O I：

10.1016/0020-7519(93)90150-W

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A detergent extract of adult Haemonchus contortus enriched in the integral membrane protein H11, previously shown to give protective immunity against the parasite, was fractionated by lectin and ion-exchange chromatography. The fractions were evaluated for their ability to immunize Clun Forest and Dorset Horn sheep against experimental haemonchosis. Most of the protective activity was associated with H11. Used in an approximately 95% pure form it gave a mean reduction in parasite egg output of 94.6% and reduced male and female worm numbers by 86.5 and 93.5%, respectively. Level of protection correlated with serum antibody titre to H11.

引用

页码：271 / 280

页数：10

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