DETERMINATION OF MK-0476 IN HUMAN PLASMA BY LIQUID-CHROMATOGRAPHY

被引：31

作者：

AMIN, RD ^{[1
]}

CHENG, HY ^{[1
]}

ROGERS, JD ^{[1
]}

机构：

[1] MERCK SHARP & DOHME LTD,RES LABS,DEPT DRUG METAB,W POINT,PA 19486

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1995年 / 13卷 / 02期

关键词：

MK-0476; LEUKOTRIENE; D-4; RECEPTOR; ANTAGONIST; LC; ASSAY METHOD; HUMAN PLASMA;

D O I：

10.1016/0731-7085(94)00138-R

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and accurate assay for quantitating MK-0476 [sodium 1-(((1(R)-(3-(2-(7-chloro-2-quinolinyl)-(E)-ethenyl)phenyl)(3-(2-(1-hydroxy-1-methylethyl)phenyl)propyl)thio)-methyl)cyclopropane)acetale], which is a potent and selective leukotriene D-4-receptor antagonist, in human plasma has been developed. The method involves precipitation of protein and reversed-phase liquid chromatography with fluorescence detection. The assay is linear in the range of 30-3000 ng ml(-1) of MK-0476, and the limit of detection is 5 ng ml(-1). The interday precision (% relative standard deviation) values of this method al 51 and 2040 ng ml(-1) are 10 and 3%, respectively. The interday accuracy values al these concentrations are 94 and 104%, respectively. The absolute recovery of MK-0476 is 99%. The utility of this method to determine plasma concentrations of MK-0476 in humans receiving the drug orally was demonstrated.

引用

页码：155 / 158

页数：4