CLONING AND CHARACTERIZATION OF THE METHYL COENZYME-M REDUCTASE GENES FROM METHANOBACTERIUM-THERMOAUTOTROPHICUM

被引：62

作者：

BOKRANZ, M ^{[1
]}

BAUMNER, G ^{[1
]}

ALLMANSBERGER, R ^{[1
]}

ANKELFUCHS, D ^{[1
]}

KLEIN, A ^{[1
]}

机构：

[1] UNIV MARBURG, FACHBEREICH BIOL, D-3550 MARBURG, FED REP GER

来源：

JOURNAL OF BACTERIOLOGY | 1988年 / 170卷 / 02期

关键词：

D O I：

10.1128/jb.170.2.568-577.1988

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The genes coding for methyl coenzyme M reductase were cloned from a genomic library of Methanobacterium thermoautotrophicum Marburg into Escherichia coli by using plasmid expression vectors. When introduced into E. coli, the reductases genes were expressed, yielding polypeptides identical in size to the three known subunits of the isolated enzyme, .alpha., .beta., and .gamma.. The polypeptides also reacted with the antibodies raised against the respective enzyme subunits. In M. thermoautotrophicum, the subunits are encoded by a gene cluster whose transcript boundaries were mapped. Sequence analysis revealed two more open reading frames of unknown function located between two of the methyl coenzyme M reductase genes.

引用

页码：568 / 577

页数：10

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