CHROMATOGRAPHY OF CONJUGATED BILE ACIDS ON SILICA GEL-IMPREGNATED GLASS FIBER PAPER

被引：8

作者：

GALLOTORRES, HE

HAMILTON, JG

机构：

[1] Nutrition and Metabolism Research Laboratory, Departments of Medicine and Biochemistry, Tulane University School of Medicine, New Orleans, LA

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1969年 / 7卷 / 08期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1093/chromsci/7.8.513

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Three new solvent systems are described for the separation of free and conjugated bile acids by glass fiber paper chromatography. An acidic solvent system consisting of isooctane-isopropyl ether-glacial acetic acid-isopropyl alcohol, 1:1:1:1: (v/v) was found suitable for the separation of glycineconjugated bile acids. A more polar modification of this acidic solvent system with chloroform substituting for isooctane, was found adequate for the separation of taurine conjugates. A basic solvent system consisting of chloroformisopropyl alcohol-ammonium hydroxide, 20:25:1 was found satisfactory for the separation of glycine and taurine-conjugated bile acids, and lecithin. © 1969, Oxford University Press. All rights reserved.

引用

页码：513 / +

页数：1

共 5 条

[1]

GREGG JA, 1966, J LIPID RES, V7, P579

[2] EFFECT OF ORAL NEOMYCIN ON CONVERSION OF CHOLIC ACID TO DEOXYCHOLIC IN MAN [J].

HAMILTON, JG .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1963, 101 (01) :7-&

[3] A SILICA GEL IMPREGNATED GLASS FIBER FILTER PAPER AND ITS USE FOR SEPARATION OF CHOLESTEROL, TRIGLYCERIDES AND CHOLESTERYL AND METHYL ESTERS OF FATTY ACIDS [J].

HAMILTON, JG ;

MULDREY, JE ;

SWARTWOUT, JR ;

MILLER, ON .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1961, 5 (03) :226-&

[4] DETECTION OF BILE ACIDS IN THIN-LAYER CHROMATOGRAPHY [J].

KRITCHEVSKY, D ;

ROTHBLAT, GH ;

MARTAK, DS .

ANALYTICAL BIOCHEMISTRY, 1963, 5 (05) :388-&

[5]

SUBBIAH MT, 1968, J LIPID RES, V9, P288

← 1 →