DETECTION OF BACILLUS-CEREUS FLAGELLAR ANTIGEN BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA)

被引：12

作者：

MURAKAMI, T

HIRAOKA, K

MIKAMI, T

MATSUMOTO, T

KATAGIRI, S

SUZUKI, M

机构：

[1] TOHOKU COLL PHARMACEUT SCI,DEPT MICROBIOL,SENDAI,MIYAGI 981,JAPAN

[2] BANYU PHARMACEUT CO LTD,QUAL CONTROL LAB,OOSATO,SAITAMA 36002,JAPAN

[3] YAMAGATA PREFECTURAL PUBL HLTH LABS,YAMAGATA 990,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1991年 / 35卷 / 03期

关键词：

D O I：

10.1111/j.1348-0421.1991.tb01551.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A serological typing scheme of Bacillus cereus has been developed by immunochemical analyses of flagellar antigen using an agglutination method. Enzyme-linked immunosorbent assay (ELISA) for the classification of flagellar serotype of Bacillus cereus had greater sensitivity, 10-500 times, than that of agglutination method. The specificity of flagellar antigen and antibody was determined by immunogold electron microscopy and ELISA inhibition assay. Application of ELISA is useful for the detection of the small amounts and many kinds of antigen-antibody reactions.

引用

页码：223 / 234

页数：12

共 16 条

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