ANALYSIS OF DIAZEPAM AND CHLORDIAZEPOXIDE AND THEIR RELATED-COMPOUNDS USING SUPERCRITICAL FLUID CHROMATOGRAPHY

The analysis of diazepam, chlordiazepoxide and their by- and degradation products using supercritical fluid chromatography (SFC) was investigated. The separations were carried out using a capillary SB-cyanopropyl-50 column with carbon dioxide as mobile phase and flame ionization detection. Typical analysis time was in the range of 20-23 min. Accuracy and precision of the SFC method were both in the 1-4% range. The SFC method was then applied to dosage forms containing diazepam and chlordiazepoxide. The data obtained using the SFC methods was compared with HPLC methods.