CELLULASE-FREE XYLANASE PRODUCTION FROM AN ALKALOPHILIC BACILLUS SPECIES

被引：31

作者：

BALAKRISHNAN, H ^{[1
]}

DUTTACHOUDHURY, M ^{[1
]}

SRINIVASAN, MC ^{[1
]}

RELE, MV ^{[1
]}

机构：

[1] NATL CHEM LAB,DEPT BIOCHEM SCI,POONA 411008,MAHARASHTRA,INDIA

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1992年 / 8卷 / 06期

关键词：

ALKALOPHILIC BACILLUS; CELLULASE-FREE XYLANASE; HEMICELLULASE;

D O I：

10.1007/BF01238802

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An alkalophilic Bacillus (NCL-87-6-10, NCIM 2128), with a high productivity for extracellular xylanase (EC 3.2.1.8) and free of cellulase, was isolated from soil containing coconut fibre detritus. When grown on a wheat bran/yeast extract medium in submerged culture for 48 h, it produced 100 to 120 IU of enzyme activity per ml. The crude enzyme consists of two fractions of apparent mol sizes of approx 10.4 and 29 kDa in the proportion of 90: 10, as determined by native gel exclusion chromatography. Optimum activity of the xylanase was at 60-degrees-C and pH 8.0. A two-fold increase in enzyme activity was obtained when reducing agents, thioethanol and dithiothreitol, were included in the assay.

引用

页码：627 / 631

页数：5

共 20 条

[1] Andrews P., The gel filtration behaviour of proteins related to their molecular weights over a wide range, Biochemical Journal, 96, pp. 595-606, (1965)
[2] Bastawde K.B., Tabatabai L.B., Meagher M.M., Srinivasan M.C., Vartak H.G., Rele M.V., Reilly P.J., Catalytic properties and partial amino acid sequence of an actinomycete endo-(1-4)-β- d-xylanase from Chainia species, American Chemical Society Symposium Series, pp. 418-425, (1990)
[3] Berghem E.R., Petterson L.G., The mechanism of enzymatic cellulose degradation, European Journal of Biochemistry, 37, pp. 21-30, (1973)
[4] Bernfeld P., Amylases
[5] α and β, Methods in Enzymology, 1, pp. 149-158, (1955)
[6] Clarke A.J., Yaguchi M., The role of carboxyl groups in the function of endo-β-1,4-glucanase from Schizophyllum commune, European Journal of Biochemistry, 149, pp. 233-238, (1985)
[7] Collmer A., Riad J.L., Mount M.S., Assay methods for pectic enzymes, Methods in Enzymology, 161, pp. 333-335, (1988)
[8] Davis B.J., Disc electrophoresis-II: Method and Application to human serum proteins, Annals of New York Academy of Sciences, 121, pp. 404-427, (1964)
[9] Eriksson K.E., Petterson G., Studies on cellulolytic enzymes. Some structural properties of the cellulase from Penicillium notatum, Archives of Biochemistry and Biophysics, 124, pp. 160-166, (1968)
[10] Grabski A.C., Jeffries T.W., Production, purification and characterization of β-(1-4)-endoxylanase of Streptomyces roseiscleroticus, Applied and Environmental Microbiology, 57, pp. 987-992, (1991)

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