MUTATIONAL STUDY OF THE ALCOHOL DEHYDROGENASE-1 FCM DUPLICATION IN MAIZE

被引:10
作者
BIRCHLER, JA [1 ]
SCHWARTZ, D [1 ]
机构
[1] INDIANA UNIV,DEPT BIOL,BLOOMINGTON,IN 47401
关键词
alcohol dehydrogenase; electrophoretic mobility mutants; gene duplication; maize;
D O I
10.1007/BF00504355
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The alcohol dehydrogenase-1 FCm (Adh-FCm) duplication in maize was subjected to ethyl methanesulfonate (EMS) mutagenesis. Of the mutants recovered, eight produced ADH polypeptides with altered electrophoretic mobility. Four produced new mobilities of the progenitor F with no change of the Cm molecule; the remainder altered only the Cm enzyme. No cases were found in which the electrophoretic mobilities of the two types of subunits were simultaneously altered, and no complete nulls lacking both F and Cm were recovered. These observations confirm the duplicate nature of the FCm complex. © 1979 Plenum Publishing Corporation.
引用
收藏
页码:1173 / 1180
页数:8
相关论文
共 10 条
[1]   GENETIC RECOMBINATION WITH ETHYL-METHANESULPHONATE-INDUCED WAXY MUTANTS IN-MAIZE [J].
BRIGGS, RW ;
AMANO, E ;
SMITH, HH .
NATURE, 1965, 207 (4999) :890-&
[2]   DISSOCIATION AND REASSOCIATION OF MAIZE ALCOHOL-DEHYDROGENASE - ALLELIC DIFFERENCES IN REQUIREMENT FOR ZINC [J].
FISCHER, M ;
SCHWARTZ, D .
MOLECULAR & GENERAL GENETICS, 1973, 127 (01) :33-38
[3]  
FREELING M, 1976, GENETICS, V83, P701
[4]  
JOHNSON GB, 1977, GENETICS, V87, P139
[5]  
LAUGHNER WJ, 1970, THESIS INDIANA U
[6]  
Ohno S, 1970, EVOLUTION GENE DUPLI
[7]   A MOLECULAR BASIS FOR HETEROSIS [J].
SCHWARTZ, D ;
LAUGHNER, WJ .
SCIENCE, 1969, 166 (3905) :626-&
[8]   ALCOHOL DEHYDROGENASE IN MAIZE - GENETIC BASIS FOR MULTIPLE ISOZYMES [J].
SCHWARTZ, D .
SCIENCE, 1969, 164 (3879) :585-&
[9]  
SCHWARTZ D, 1966, GENETICS, V53, P709
[10]   SINGLE GENE HETEROSIS FOR ALCOHOL-DEHYDROGENASE IN MAIZE - NATURE OF SUBUNIT INTERACTION [J].
SCHWARTZ, D .
THEORETICAL AND APPLIED GENETICS, 1973, 43 (3-4) :117-120