PROTEOLYTIC ACTIVATION AND INACTIVATION OF CHITIN SYNTHASE FROM NEUROSPORA-CRASSA

被引：21

作者：

ARROYOBEGOVICH, A

RUIZHERRERA, J

机构：

[1] UNIV NACL AUTONOMA MEXICO, INST BIOL, DEPT BIOL EXPTL, MEXICO CITY 20, MEXICO

[2] NATL POLYTECH INST, CTR INVEST & ESTUDIOS AVANZADOS, DEPT GENET & BIOL MOLEC, MEXICO CITY 14, MEXICO

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1979年 / 113卷 / AUG期

关键词：

D O I：

10.1099/00221287-113-2-339

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

The proteolytic activation and inactivation of chitin synthase from N. crassa was studied. Chitin synthase was found mostly in an inactive form which could be activated either endogenously or by the addition of exogenous proteases. The addition of protease inhibitors prevented the endogenous activation of the enzyme. The stability of the inactive enzyme improved when buffers of pH values between 8.2-9.5 were employed. The inactive enzyme was mainly associated with membranous fractions.

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页码：339 / 345

页数：7

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