E-TYPE DELAYED FLUORESCENCE DEPOLARIZATION, A TECHNIQUE TO PROBE ROTATIONAL MOTION IN THE MICROSECOND RANGE

被引：38

作者：

GREINERT, R

STAERK, H

STIER, A

WELLER, A

机构：

[1] Max-Planck-Institut für biophysikalische Chemie

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1979年 / 1卷 / 02期

关键词：

cytochrome P-450; delayed fluorescence; fluorescence depolarization; membrane proteins; rotational motion;

D O I：

10.1016/0165-022X(79)90014-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

E (eosin)-type delayed fluorescence depolarization studies extend the time range for the measurement of rotational diffusion to μs and ms, thereby allowing investigation of slow rotational movement of macromolecules like membrane proteins. An apparatus is described for the determination of time-dependent anisotropy in this interesting time range. The method has been tested on eosin-labelled cytochrome P-450 incorporated into phospholipid membrane vesicles. © 1979.

引用

页码：77 / 83

页数：7

共 15 条

[1]

BOSTERLING B, MOL PHARMACOL

[2]

CHEN LA, 1977, J BIOL CHEM, V252, P2163

[3]

Cherry R J, 1978, Methods Enzymol, V54, P47

[4] ROTATIONAL AND TRANSLATIONAL DIFFUSION IN MEMBRANES [J].