NATURAL HUMAN-IGG INHIBITS THE PRODUCTION OF TUMOR-NECROSIS-FACTOR-ALPHA AND INTERLEUKIN-1-ALPHA THROUGH THE FC PORTION

被引：10

作者：

HORIUCHI, A ^{[1
]}

ABE, Y ^{[1
]}

MIYAKE, M ^{[1
]}

OSUKA, Y ^{[1
]}

KIMURA, S ^{[1
]}

机构：

[1] EHIME UNIV,SCH MED,DEPT SURG 2,SHIGENOBU,EHIME 79102,JAPAN

来源：

SURGERY TODAY-THE JAPANESE JOURNAL OF SURGERY | 1993年 / 23卷 / 03期

关键词：

IGG; FRAGMENTS; TNF-ALPHA; IL-1-ALPHA; PRODUCTION; PBMC;

D O I：

10.1007/BF00309234

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

The overproduction of cytokines such as the tumor necrosis factor-alpha (TNF-alpha) and interleukin-1alpha (IL-1alpha) may cause further deterioration in the already critical condition of patients with shock, sepsis, and acute inflammation. The effectiveness of infusion therapy of natural human IgG to such patients is suggested to depend partly upon the inhibition of the productivity of these cytokines. In this study, we investigated the modulation effects of IgG and its fragments on the production of TNF-alpha and IL-1alpha, on human peripheral blood mononuclear cells (PBMC). The production of TNF-alpha and IL-1alpha was found to be dose-dependently inhibited by IgG when stimulated by lipopolysaccharide (LPS), phytohemagglutinin (PHA), concanavalin A (Con A), and interleukin-2 (IL-2). However, no inhibition was seen when stimulated by phorbormyristate acetate (PMA). The F(ab')2 fragment showed enhancing effects on cytokine production by LPS, while the Fc fragment showed not as much inhibitory effect as whole intact IgG. IgG showed no direct cytotoxic effect on PBMC. These data suggest that natural human IgG inhibits TNF-alpha and IL-1alpha production by PBMC through the Fc portion. The results of this study led us to conclude that whole intact IgG may be the best form of therapeutic delivery.

引用

页码：241 / 245

页数：5

共 20 条

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