STUDY AND OPTIMIZATION OF CITRUS FLAVANONE AND FLAVONES ELUCIDATION BY REVERSE-PHASE HPLC WITH SEVERAL MOBILE PHASES - INFLUENCE OF THE STRUCTURAL CHARACTERISTICS

被引：28

作者：

CASTILLO, J ^{[1
]}

BENAVENTEGARCIA, O ^{[1
]}

DELRIO, JA ^{[1
]}

机构：

[1] UNIV MURCIA,FAC BIOL,DEPT PLANT BIOL,E-30071 MURCIA,SPAIN

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 07期

关键词：

D O I：

10.1080/10826079408013175

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic procedure for the analysis of the different flavonoids found in Citrus aurantium tissue extracts has been developed. It employs a C18 reverse phase column and an elution isocratic-gradient system with a mixture of water, methanol, acetonitrile and acetic acid. We make an exhaustive description of the optimisation process by studying the quantitative chromatographic parameters: k', w, alpha, N, HETP and R. The use of different extraction solvents for flavonoids in Citrus aurantium tissues was also studied, DMSO presenting the best results. Twelve compounds were detected, isolated and their spectral structures characterized.

引用

页码：1497 / 1523

页数：27

共 33 条

[1]

BAUMANN F, 1971, BASIC LIQUID CHROMAT

[2]

BERNARDINI M, 1984, J CHROMATOGR, V284, P269

[3] NARINGIN AND NEOHESPERIDIN LEVELS DURING DEVELOPMENT OF LEAVES, FLOWER BUDS, AND FRUITS OF CITRUS-AURANTIUM [J].

CASTILLO, J ;

BENAVENTE, O ;

DELRIO, JA .

PLANT PHYSIOLOGY, 1992, 99 (01) :67-73

[4] ANALYSIS OF COMMERCIAL HESPERIDIN METHYL CHALCONE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

CASTILLO, J ;

BENAVENTE, O ;

BORREGO, F ;

DELRIO, JA .

JOURNAL OF CHROMATOGRAPHY, 1991, 555 (1-2) :285-290

[5] NEODIOSMIN, A FLAVONE GLYCOSIDE OF CITRUS-AURANTIUM [J].

DELRIO, JA ;

BENAVENTE, O ;

CASTILLO, J ;

BORREGO, F .

PHYTOCHEMISTRY, 1992, 31 (02) :723-724

[6] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR QUANTITATION OF HESPERIDIN IN ORANGE JUICE [J].

FISHER, JF .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1978, 26 (06) :1459-1460

[7]

FISHER JF, 1977, P INT SOC CITRICULTU, V3, P813

[8] USE OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY IN THE SEPARATION OF FLAVONOL GLYCOSIDES AND FLAVONOL SULFATES [J].

HARBORNE, JB ;

BOARDLEY, M .

JOURNAL OF CHROMATOGRAPHY, 1984, 299 (02) :377-385

[9] CINNAMATE HYDROXYLATION AND ENZYMES LEADING FROM PHENYLPYRUVATE TO P-COUMARATE SYNTHESIS IN GRAPEFRUIT TISSUES [J].

HASEGAWA, S ;

MAIER, VP .

PHYTOCHEMISTRY, 1972, 11 (04) :1365-+

[10] STUDIES ON FLAVANONE GLYCOSIDES .4. THE GLYCOSIDES OF RIPE FRUIT PEEL AND FLOWER PETALS OF CITRUS-AURANTIUM L [J].

HATTORI, S ;

SHIMOKORIYAMA, M ;

KANAO, M .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1952, 74 (14) :3614-3615

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