MOLECULAR-CLONING OF POLYMORPHIC MARKERS ON RLGS GEL USING THE SPOT TARGET CLONING METHOD

被引：37

作者：

HIROTSUNE, S

SHIBATA, H

OKAZAKI, Y

SUGINO, H

IMOTO, H

SASAKI, N

HIROSE, K

OKUIZUMI, H

MURAMATSU, M

PLASS, C

CHAPMAN, VM

TAMATSUKURI, S

MIYAMOTO, C

FURUICHI, Y

HAYASHIZAKI, Y

机构：

[1] INST PHYS & CHEM RES,TSUKUBA LIFE SCI CTR,GENE BANK,3-1-1 KOYADAI,TSUKUBA,IBARAKI 305,JAPAN

[2] ROSWELL PK CANC INST,DEPT MOLEC & CELLULAR BIOL,BUFFALO,NY 14263

[3] NIPPON ROCHE RES CTR,DEPT MOLEC GENET,KAMAKURA,KANAGAWA 247,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 194卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1981

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method for target cloning of DNA fragments corresponding to spots on the two-dimensional restriction landmark genomic scanning (RLGS) profile has been developed (targeted spot cloning). We used a Not I restriction trapper to select target DNA fragments from Not I, Eco RV double digests of genomic DNA. The use of the restriction trapper substantially reduces the background clones that are established from the direct recovery of RLGS spot DNA from the two-dimensional gels. Genomic DNA clones were isolated in this study as mouse genome markers for 58 spot loci that were previously characterized using RLGS spot mapping. This method provides a powerful tool for isolating DNA clones after their identification by RLGS system. © 1993 Academic Press, Inc.

引用

页码：1406 / 1412

页数：7

共 8 条

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