EFFICIENT OPTIMIZATION OF ELISAS

被引：10

作者：

REIKEN, SR

VANWIE, BJ

SUTISNA, H

KURDIKAR, DL

DAVIS, WC

机构：

[1] WASHINGTON STATE UNIV,DEPT CHEM ENGN,PULLMAN,WA 99164

[2] WASHINGTON STATE UNIV,DEPT VET MICROBIOL & PATHOL,PULLMAN,WA 99164

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1994年 / 177卷 / 1-2期

关键词：

ELISA; OPTIMIZATION; FRACTIONAL FACTORIAL DESIGN;

D O I：

10.1016/0022-1759(94)90157-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper, we describe a technique for using the statistical method of fractional factorial design in the optimization of an ELISA. Fractional factorial design dramatically reduces the total number of experiments required in the optimization. In addition, this technique enables us to determine the parameters that give the maximum sensitivity range with the most accuracy for the ELISA studied.

引用

页码：199 / 206

页数：8