3'-TERMINAL NUCLEOTIDE-SEQUENCE (N=41) OF SATELLITE TOBACCO NECROSIS VIRUS-RNA

被引：4

作者：

MERREGAERT, J ^{[1
]}

VANEMMELO, J ^{[1
]}

FIERS, W ^{[1
]}

机构：

[1] STATE UNIV GHENT, MOLEK BIOL LAB, B-9000 GHENT, BELGIUM

来源：

VIROLOGY | 1979年 / 98卷 / 01期

关键词：

D O I：

10.1016/0042-6822(79)90536-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To convert satellite tobacco necrosis virus RNA into a template for complementary (c) DNA synthesis, a poly(A) tail was added to its 3''-end by Escherichia coli ATP:RNA adenyltransferase. This product could then be copied by avian myeloblastosis virus RNA-dependent DNA polymerase in the presence of p(dT)10 as primer. Under appropriate conditions, cDNA was synthesized discontinously and limited transcripts were obtained when 1 or more deoxynucleoside triphosphates were omitted from the reaction mixture. Phased cDNA fragments suitable for sequence analysis were synthesized when (dT)8dG, 32P-labeled at the 5''-end, was used as a primer. The sequence of the 3''-terminal 41 nucleotides of the viral RNA is presented.

引用

页码：182 / 190

页数：9

共 30 条

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