TRYPSIN ACTIVATION OF INACTIVE RENIN - PLASMA BLANKS AND ANGIOTENSIN-I RADIOIMMUNOASSAY

被引:5
作者
BARRETT, JD [1 ]
EGGENA, P [1 ]
机构
[1] UNIV CALIF LOS ANGELES, LOS ANGELES, CA 90024 USA
关键词
PRORENIN; NEPHRECTOMY; ANGIOTENSIN-I RADIOIMMUNOASSAY; RAT; PLASMA BLANKS;
D O I
10.1139/y91-194
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Divergent conclusions exist as to whether inactive renin is present in nephrectomized rat plasma. A major factor contributing to this conflict may be related to significant changes in the "plasma blank" when trypsin-treated plasma is subjected to angiotensin I (AI) radioimmunoassay (RIA). In normal, but not nephrectomized rat plasma, AI-like substances are present in direct proportion to active renin. These substances are destroyed by trypsin. However, trypsin generates additional AI-like material, in both normal and nephrectomized rat plasma. This material, which is present in proportion to the renin substrate concentration, does not appear to be tetradecapeptide (TDP). In normal plasma, however, exogenous TDP is converted to AI in proportion to the active renin concentration and AI generation from TDP is increased by activation of inactive renin. However, in nephrectomized rat plasma, no AI generation from TDP was evident either before or after trypsin treatment. The coincident tryptic generation of a substance that quenches the levels of AI detected by RIA, combined with significant changes in the levels of endogenous and trypsin generated AI-like substances, may have significant bearing on the measured levels of inactive renin.
引用
收藏
页码:1315 / 1320
页数:6
相关论文
共 16 条
[1]  
BARRETT JD, 1988, J HYPERTENS, V6, P49
[2]   INFLUENCE OF ANGIOTENSINASE INHIBITORS ON ENZYMATIC-ACTIVITY OF RENIN [J].
BARRETT, JD ;
EGGENA, P ;
SAMBHI, MP .
BIOCHEMICAL MEDICINE, 1976, 16 (02) :157-168
[3]   EFFECTS OF STIMULATION OF RENIN RELEASE ON TRYPSIN-ACTIVABLE RENIN IN RAT PLASMA [J].
BARRETT, JD ;
EGGENA, P ;
SOWERS, JR ;
SAMBHI, MP .
AMERICAN JOURNAL OF PHYSIOLOGY, 1982, 243 (03) :E206-E212
[4]   INDEPENDENT CHANGE OF PLASMA AND TISSUE RENIN IN RESPONSE TO ANESTHETICS [J].
BARRETT, JD ;
EGGENA, P ;
KRALL, JF .
CLINICAL AND EXPERIMENTAL HYPERTENSION PART A-THEORY AND PRACTICE, 1988, 10 (05) :757-765
[5]   TRYPTIC GENERATION OF AN ANGIOTENSIN BINDING SUBSTANCE [J].
BARRETT, JD ;
EGGENA, P .
CLINICAL AND EXPERIMENTAL HYPERTENSION PART A-THEORY AND PRACTICE, 1987, 9 (11) :1823-1841
[6]  
DOY Y, 1984, HYPERTENSION, V6, P816
[7]   VALIDITY OF COMPARING MEASUREMENTS OF ANGIOTENSIN-I GENERATED IN HUMAN PLASMA BY RADIOIMMUNOASSAY AND BIOASSAY [J].
EGGENA, P ;
BARRETT, JD ;
SAMBHI, MP ;
WIEDEMAN, CE .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1974, 39 (05) :865-870
[8]   REGULATED SYSTEMIC ACTIVATION OF RAT PLASMA PRORENIN [J].
HARE, GMT ;
IOANNOU, P ;
DUBISKI, S ;
OSMOND, DH .
HYPERTENSION, 1987, 10 (01) :122-126
[9]   PREPARATION OF IODINE-131 LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC ACTIVITY [J].
HUNTER, WM ;
GREENWOOD, FC .
NATURE, 1962, 194 (4827) :495-&
[10]   DOES ANGIOTENSIN REGULATE SYSTEMIC CONVERSION OF PRORENIN TO RENIN [J].
IOANNOU, P ;
ISMAIL, R ;
LOH, AY ;
OSMOND, DH .
CLINICAL AND EXPERIMENTAL HYPERTENSION PART A-THEORY AND PRACTICE, 1989, 11 (07) :1273-1300