INHIBITION OF O-2-. GENERATING OXIDASE OF NEUTROPHILS BY IODONIUM BIPHENYL IN A CELL FREE SYSTEM - EFFECT OF THE REDOX STATE OF THE OXIDASE COMPLEX

被引：26

作者：

DOUSSIERE, J

VIGNAIS, PV

机构：

[1] Laboratoire de Biochimie, Département de Biologie Moléculaire et Structurale, Centre d'Etudes Nucléaires, 38041 Grenoble cedex

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 175卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(05)81212-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conditions of inhibition of neutrophil O2{minus sign, dot below} generating oxidase by iodonium biphenyl (IBP) were studied. In a cell free system of oxidase activation consisting of neutrophil membranes and cytosol, GTP-γ-S, Mg2+ and arachidonic acid, the inhibitory effect of IBP depended on the redox conditions of the medium. Inhibition was observed when the medium was supplemented with dithionite or NADPH. When the cell free system was incubated with IBP in the absence of reducing agents, full oxidase activity was recovered after removal of free IBP by gel filtration. Bovine neutrophil membranes, but not cytosol, contained component(s) sensitive to IBP. Upon treatment of neutrophil membranes by IBP followed by reduction, the spectrum of reduced cytochrome b558 was modified, suggesting that cytochrome b558 is a target site for IBP. © 1991 Academic Press, Inc.

引用

页码：143 / 151

页数：9

共 15 条

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