CLUSTERIN ENHANCES THE FORMATION OF INSOLUBLE IMMUNE-COMPLEXES

被引：47

作者：

WILSON, MR ^{[1
]}

ROETH, PJ ^{[1
]}

EASTERBROOKSMITH, SB ^{[1
]}

机构：

[1] UNIV SYDNEY,DEPT BIOCHEM,SYDNEY,NSW 2006,AUSTRALIA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 177卷 / 03期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0006-291X(91)90635-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Clusterin was purified from human serum by sequential affinity chromatography over IgG-, protein A- and Con A-Sepharose. The protein was ∼ 70 kDa by SDS/PAGE under non-reducing conditions and was resolved into ∼ 35 kDa bands under reducing conditions. The protein reacted with clusterin-specific Mabs in ELISA and in Western blots. Its N-terminal sequences agreed with those published for clusterin. An antiserum specific for clusterin made by the above method detected it in complement membrane attack complexes on rabbit erythrocyte membranes. The interaction of clusterin with IgG was physiologically relevant because it was found to increase the rate of formation of insoluble immune complexes. © 1991.

引用

页码：985 / 990

页数：6

共 18 条

[1]

AHMED AEE, 1989, IMMUNOLOGY, V66, P20

[2]

AHMED AEE, 1988, IMMUNOLOGY, V64, P45

[3]

BLASCHUK O, 1983, J BIOL CHEM, V258, P7714

[4] SANDWICH ELISA ASSAY FOR QUANTITATIVE MEASUREMENT OF SP-40,40 IN SEMINAL PLASMA AND SERUM [J].