LIPIDS, PROTEINS, AND STRUCTURE OF SEED OIL BODIES FROM DIVERSE SPECIES

被引:454
作者
TZEN, JTC [1 ]
CAO, YZ [1 ]
LAURENT, P [1 ]
RATNAYAKE, C [1 ]
HUANG, AHC [1 ]
机构
[1] UNIV CALIF RIVERSIDE,DEPT BOT & PLANT SCI,RIVERSIDE,CA 92521
关键词
D O I
10.1104/pp.101.1.267
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Oil bodies isolated from the mature seeds of rape (Brassica napus L.), mustard (Brassica juncea L.), cotton (Gossypium hirsutum L.), flax (Linus usitatis simum), maize (Zea mays L), peanut (Arachis hypogaea L.), and sesame (Sesamum indicum L) had average diameters that were different but within a narrow range (0.6-2.0 mum), as measured from electron micrographs of serial sections. Their contents of triacylglycerols (TAG), phospholipids, and proteins (oleosins) were correlated with their sizes. The correlation fits a formula that describes a spherical particle surrounded by a shell of a monolayer of phospholipids embedded with oleosins. Oil bodies from the various species contained substantial amounts of the uncommon negatively charged phosphatidylserine and phosphatidylinositol, as well as small amounts of free fatty acids. These acidic lipids are assumed to interact with the basic amino acid residues of the oleosins on the surface of the phospholipid layer. Isoelectrofocusing revealed that the oil bodies from the various species had an isoelectric point of 5.7 to 6.6 and thus possessed a negatively charged surface at neutral pH. We conclude that seed oil bodies from diverse species are very similar in structure. In rapeseed during maturation, TAG and oleosins accumulated concomitantly. TAG-synthesizing acyltransferase activities appeared at an earlier stage and peaked during the active period of TAG accumulation. The concomitant accumulation of TAG and oleosins is similar to that reported earlier for maize and soybean, and the finding has an implication for the mode of oil body synthesis during seed maturation.
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页码:267 / 276
页数:10
相关论文
共 26 条
[1]  
Appleqvist L.-A., 1975, Recent advances in the chemistry and biochemistry of plant lipids. Proceedings of a symposium arranged by the Phytochemical Society and the Lipid Group of the Biochemical Society, University of East Anglia, Norwich, April, 1974., P247
[2]   ACYL COENZYME-A PREFERENCE OF DIACYLGLYCEROL ACYLTRANSFERASE FROM THE MATURING SEEDS OF CUPHEA, MAIZE, RAPESEED, AND CANOLA [J].
CAO, YZ ;
HUANG, AHC .
PLANT PHYSIOLOGY, 1987, 84 (03) :762-765
[3]  
CUMMINS I, 1990, PLANT LIPID BIOCHEMISTRY, STRUCTURE AND UTILIZATION, P231
[4]  
Dittmer J, 1969, METHOD ENZYMOL, V14, P482, DOI [10.1016/S0076-6879(69)14055-0, DOI 10.1016/S0076-6879(69)14055-0]
[5]  
FAWCETT T, 1990, PLANT LIPID BIOCHEMISTRY, STRUCTURE AND UTILIZATION, P172
[7]   OIL BODIES AND OLEOSINS IN SEEDS [J].
HUANG, AHC .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1992, 43 :177-200
[8]  
MOHANKUMAR C, 1990, J AM OIL CHEM SOC, V10, P665
[9]  
MUDD JB, 1980, BIOCH PLANTS, V4, P249
[10]   A CLASS OF AMPHIPATHIC PROTEINS ASSOCIATED WITH LIPID STORAGE BODIES IN PLANTS - POSSIBLE SIMILARITIES WITH ANIMAL SERUM APOLIPOPROTEINS [J].
MURPHY, DJ ;
KEEN, JN ;
OSULLIVAN, JN ;
AU, DMY ;
EDWARDS, EW ;
JACKSON, PJ ;
CUMMINS, I ;
GIBBONS, T ;
SHAW, CH ;
RYAN, AJ .
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1088 (01) :86-94