TRIPLOID AND HEXAPLOID REGENERANTS FROM HEXAPLOID TIMOTHY (PHLEUM-PRATENSE L) VIA ANTHER CULTURE

被引：7

作者：

ABDULLAH, AA ^{[1
]}

PEDERSEN, S ^{[1
]}

ANDERSEN, SB ^{[1
]}

机构：

[1] ROYAL VET & AGR UNIV,DEPT AGR SCI,PLANT BREEDING & BIOTECHNOL SECT,DK-1871 FREDERIKSBERG C,DENMARK

来源：

PLANT BREEDING | 1994年 / 112卷 / 04期

关键词：

PHLEUM-PRATENSE; AUTOHEXAPLOID-TRIPLOID-HAPLOID; ANTHER CULTURE;

D O I：

10.1111/j.1439-0523.1994.tb00694.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Pollen embryos were obtained from eight genotypes and viable green plants were regenerated from four genotypes in an anther-culture experiment with 165 genotypes of Phleum pratense L. Formation of proembryos inside the cultured anthers during the first 10-12 days was significantly influenced by genotypes and by the type of nutrient media. Primary embryos developed into multiple secondary embryos before regeneration of plants. Among a total of 62 plants regenerated, only 13 were albinos. Of the green regenerants, 11 were triploid while 35 were hexaploid when DNA-content was measured by flow cytometry. Eight plants with a triploid DNA-content did possess the triploid chromosome number of 21. Triploid and hexaploid regenerants from two different parents showed simplified isozyme (GPI and PGD) banding patterns relative to that of their parents.

引用

页码：342 / 345

页数：4

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