SIMULTANEOUS DETERMINATION OF RETINYL ESTERS AND RETINOL IN HUMAN LIVERS BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：18

作者：

GOT, L ^{[1
]}

GOUSSON, T ^{[1
]}

DELACOUX, E ^{[1
]}

机构：

[1] HOP BEAUJON, SERV BIOCHIM, F-92110 CLICHY, FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1995年 / 668卷 / 02期

关键词：

D O I：

10.1016/0378-4347(95)00087-Y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the simultaneous determination of retinol an? retinyl esters in human liver samples is presented. The free retinol and the prevalent retinyl esters (retinyl palmitate, oleate and stearate) are resolved within less than 30 min, using an octasilyl (C-8)-substituted column and an isocratic elution with methanol-water as mobile phase. This method allows to determine in duplicate all retinyl ester concentrations in small liver samples (3-10 mg of fresh tissue). The results obtained from thirteen patients-without liver disease are described.

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页码：233 / 239

页数：7

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