CARBOXYLESTERASES (EC 3.1.1) . PURIFICATION AND TITRATION OF OX LIVER CARBOXYLESTERASE

Ox liver carboxylesterase has been reproducibly purified on a large scale from an acetone powder. The enzyme, while significantly purified, is not electrophoretically homogeneous and consists of three major proteins, here called electrophoretic variants 1, 2, and 3. All three variants are very similar protein molecules, apparently distinguished by small charge differences. The equivalent weight of the enzyme has been estimated at ~68,000 based on titration with o- and p-nitrophenyl dimethylcarbamates and p-nitrophenyl diethyl phosphate. © 1969, American Chemical Society. All rights reserved.