SUBCELLULAR-DISTRIBUTION OF GABA RECEPTORS IN BOVINE RETINA

被引:34
作者
REDBURN, DA
KYLES, CB
FERKANY, J
机构
[1] Department of Neurobiology and Anatomy, The University of Texas Medical School at Houston, Houston
关键词
GABA; neurotransmitter receptors; photoreceptor terminals; retina; retinal neurotransmitters;
D O I
10.1016/0014-4835(79)90040-X
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Two synaptosomal fractions were isolated from bovine retina and analyzed for GABA receptors using a [3H]GABA binding assay. Two receptor sites were identified: one with an affinity constant of 345 nm, which was uniformly distributed between the two fractions, and a second site with an affinity constant of 38 nm, which was primarily associated with the synaptosomal fraction enriched in photoreceptor cell terminals. Both sites demonstrated a high degree of pharmacologic specificity. The GABA receptor agonist, muscimol, was a more potent displacer than imidazolacetic acid or bicuculline; uptake blockers, diaminobutyric acid and β-alanine were poor displacers. These data provide further support for the role of GABA as a neurotransmitter in retina. It also suggests the presence of a significant GABAnergic system in the outer plexiform layer of the bovine retina. © 1979.
引用
收藏
页码:525 / 532
页数:8
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