ANTIGENICITY OF A PAPILLARY SEROUS CYSTADENOCARCINOMA TISSUE HOMOGENATE AND ITS FRACTIONS

被引：41

作者：

LEVI, MM

KELLER, S

MANDL, I

机构：

[1] Department of Obstetrics and Gynecology, College of Physicians and Surgeons, Columbia University, NY

[2] Francis Delafield Hospital New York, NY

来源：

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY | 1969年 / 105卷 / 06期

关键词：

D O I：

10.1016/0002-9378(69)90091-X

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Continuing our studies of the antigenicity of papillary cystadenocarcinoma of the ovary we have investigated the production of antibodies in rabbits immunized with a tissue lyophilisate. Ouchterlony double diffusion showed 4 precipitin lines, of which one cross-reacted with normal ovarian tissue, one was identical with the line developed with tissue culture cell sonicate antigen, and 2 additional lines were found only with the tumor homogenate. Immunoelectrophoresis indicated that one antigenic component had a mobility corresponding to α-globulin and one line appeared in the β-globulin region. Gel filtration on Sephadex G-200 yielded 6 fractions of which Fraction 1 and Fraction 3 were tumor specific. Fraction 1 gave one precipitin line against nonadsorbed serum to unfractionated tumor homogenate, Fraction 3 gave 2 nonidentical and distinct lines. © 1969.

引用

页码：856 / &

共 6 条

[1] DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS [J].