PHOTODYNAMIC PROPERTIES OF TOLUIDINE BLUE CHARACTERIZED BY FLUORESCENCE AND ABSORPTION-SPECTROSCOPY

被引：6

作者：

CANETE, M

VILLANUEVA, A

机构：

[1] Departamento de Biología (C-XV), Facultad de Ciencias, Universidad Autónoma de Madrid

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 1990年 / 46卷 / 08期

关键词：

D O I：

10.1016/0584-8539(90)80205-D

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Red light photolysis studies of the production of singlet oxygen by toluidine blue dye photosensitization were measured by the photo-oxidation rate of tryptophan. Absorption spectra of toluidine blue together with each of the four synthetic nucleosides of DNA have been obtained. After irradiation, only deoxyguanosine showed any noticeable reaction at neutral pH. Abreviations (see Fig. 1): TB, toluidine blue; 1O2, singlet oxygen; TRP, tryptophan; dA, 2′-deoxyadenosine; dC, 2′-deoxycytidine; dT, 2′-deoxythymidine; dG, 2′-deoxyguanosine; NaN3, sodium azide. © 1990.

引用

页码：1265 / 1267

页数：3

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