CLONING, MAPPING, AND SEQUENCING OF THE GENE ENCODING ESCHERICHIA-COLI QUINOPROTEIN GLUCOSE-DEHYDROGENASE

被引：96

作者：

CLETONJANSEN, AM ^{[1
]}

GOOSEN, N ^{[1
]}

FAYET, O ^{[1
]}

VANDEPUTTE, P ^{[1
]}

机构：

[1] CNRS, CTR RECH BIOCHIM & GENET CELLULAIRES, F-31062 TOULOUSE, FRANCE

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 11期

关键词：

D O I：

10.1128/jb.172.11.6308-6315.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Escherichia coli contains pyrroloquinoline quinone-dependent glucose dehydrogenase. We cloned and sequenced the gene (gcd) encoding this enzyme and showed that the derived amino acid sequence is highly homologos to that of the gdhA gene product of Acinetobacter calcoaceticus. Stretches of homology also exist between the amino acid sequence of E. coli glucose dehydrogenase and other pyrroloquinoline quinonedependent dehydrogenases from several bacterial species. The position of gcd on the chromosomal map of E. coli was determined to be at 3.1 min.

引用

页码：6308 / 6315

页数：8

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