HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION FOR THE DETERMINATION OF LEVODOPA, CATECHOLAMINES AND THEIR METABOLITES IN RAT-BRAIN DIALYSATES

被引：98

作者：

SARRE, S

MICHOTTE, Y

HERREGODTS, P

DELEU, D

DEKLIPPEL, N

EBINGER, G

机构：

[1] VRIJE UNIV,INST PHARMACEUT,DEPT PHARMACEUT CHEM & DRUG ANAL,LAARBEEKLAAN 103,B-1090 BRUSSELS,BELGIUM

[2] VRIJE UNIV BRUSSELS HOSP,DEPT NEUROL,B-1090 BRUSSELS,BELGIUM

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 575卷 / 02期

关键词：

D O I：

10.1016/0378-4347(92)80147-I

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic assay with electrochemical detection is described for the simultaneous determination of levodopa, 3-O-methyldopa, dopamine. dihydroxyphenylacetic acid, homovanillic acid, 3-methoxytyramine, noradrenaline, adrenaline. 3-methoxy-4-hydroxyphenylethylene glycol and 5-hydroxyindoleacetic acid in rat brain dialysates. Samples are obtained in vivo using the microdialysis technique. Microdialysis probes are placed in the brain area to be studied and neurochemicals are collected by perfusion of the probe with modified Ringer's solution. Direct injection of the dialysates allows rapid and reliable results to be obtained.

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页码：207 / 212

页数：6

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