CALCIUM-ACTIVATED POTASSIUM CHANNELS IN CHONDROCYTES

被引：34

作者：

GRANDOLFO, M ^{[1
]}

DANDREA, P ^{[1
]}

MARTINA, M ^{[1
]}

RUZZIER, F ^{[1
]}

VITTUR, F ^{[1
]}

机构：

[1] UNIV TRIESTE,IST FISIOL,I-34127 TRIESTE,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 03期

关键词：

D O I：

10.1016/0006-291X(92)91893-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence of calcium-activated potassium channels in chondrocytes of growing cartilage was tested. Results obtained with fura-2 on cultured resting chondrocytes indicate that the cells respond to an elevation of extracellular calcium concentration ( [Ca2+]o ) from 0.1 to 2 mM increasing the intracellular concentration of the ion ( [Ca2+]i ) from 117 to 187 nM. This increment may be blocked by 3μM La3+. Patch clamp experiments in cell-attached configuration showed that, when [Ca2+]i rises, the open probability (Po) of the K+ channels increases. Increments in both Po and unitary currents of the K+ channels can be obtained after applying 2.5μM A23187 with 2mM [Ca2+]o. Hence, the results demonstrate that, in chondrocytes, a class of Ca2+-activated K+ channels is present and their activity is related to an increase of [Ca2+]i. © 1992.

引用

页码：1429 / 1434

页数：6

共 14 条

[1]

BENHAM CD, 1987, CELL CALCIUM CONTROL, P45

[2] SERUM-INDUCED CYTOSOLIC CALCIUM MOVEMENTS AND MITOGENESIS IN CULTURED PREOSSEOUS CHONDROCYTES [J].