MINIATURE GLUCOSE BIOSENSOR WITH EXTENDED LINEARITY

被引:14
作者
KANAPIENIENE, JJ
DEDINAITE, AA
LAURINAVICIUS, VSA
机构
[1] Institute of Biochemistry, the Lithuanian Academy of Sciences, Vilnius
关键词
BIOSENSOR; GLUCOSE; ENZYME ELECTRODE; GLUCOSE OXIDASE; ENZYME MEMBRANE; EXTENDED LINEARITY; AMPEROMETRIC DETECTION;
D O I
10.1016/0925-4005(92)80008-L
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A rapid and simple method for the preparation of a miniature glucose sensor (enzyme electrode) has been developed. Glucose oxidase is immobilized by glutaraldehyde in albumin gel on a cellulose diacetate membrane. This membrane is directly attached to an H2O2 detecting electrode and covered with an external polyvinyl chloride membrane. The response time of the glucose electrode thus prepared is about 2 min, the calibration graph is linear for 0-30 mM glucose, and the sensor can be used repeatedly at room temperature for at least 10 days with small deterioration in response.
引用
收藏
页码:37 / 40
页数:4
相关论文
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