INVOLVEMENT OF TUMOR-NECROSIS-FACTOR-ALPHA IN INTRACELLULAR MULTIPLICATION OF LEGIONELLA-PNEUMOPHILA IN HUMAN MONOCYTES

被引：40

作者：

MATSIOTABERNARD, P ^{[1
]}

LEFEBRE, C ^{[1
]}

SEDQUI, M ^{[1
]}

CORNILLET, P ^{[1
]}

GUENOUNOU, M ^{[1
]}

机构：

[1] FAC PHARM REIMS, IMMUNOL LAB, REIMS, FRANCE

来源：

INFECTION AND IMMUNITY | 1993年 / 61卷 / 12期

关键词：

D O I：

10.1128/IAI.61.12.4980-4983.1993

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We investigated the role of tumor necrosis factor alpha (TNF-alpha) in human peripheral monocytes infected with Legionella pneumophila in vitro. Exogenous TNF-alpha significantly inhibited the intracellular multiplication of the bacterium. This effect was concentration and time dependent and was abrogated by anti-TNF antibodies. TNF-alpha levels in the culture supernatants were low but were enhanced by the addition of gamma interferon. When monocytes were cultured and infected in the presence of pentoxyphilline, a potent inhibitor of TNF-alpha synthesis, the intracellular bacterial growth was enhanced. The effect of pentoxyphilline was concentration and time dependent and was due to the inhibition of TNF-alpha production, as shown by Northern (RNA) blot hybridization of total RNA. In addition, the pentoxyphilline partially abolished the inhibitory effect of gamma interferon on bacterial intracellular multiplication. These results suggest that gamma interferon inhibits, at least partially, the intracellular multiplication of L. pneumophila by enhancing TNF-alpha synthesis.

引用

页码：4980 / 4983

页数：4

共 21 条

[1] CHARACTERIZATION OF RECEPTORS FOR HUMAN-TUMOR NECROSIS FACTOR AND THEIR REGULATION BY GAMMA-INTERFERON [J].