HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF ATRACTYLOSIDE AND CARBOXYATRACTYLOSIDE FROM ATRACTYLIS-GUMMIFERA L

被引：13

作者：

CALMES, M ^{[1
]}

CRESPIN, F ^{[1
]}

MAILLARD, C ^{[1
]}

OLLIVIER, E ^{[1
]}

BALANSARD, G ^{[1
]}

机构：

[1] FAC PHARM MARSEILLE,PHARMACOGNOSIE LAB,F-13385 MARSEILLE 5,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 663卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)80503-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A reversed-phase high-performance liquid chromatographic method is described for the identification and determination of two toxic compounds, atractyloside and carboxyatractyloside, in a 60% methanolic extract of Atractylis gummifera L. roots. Separation was achieved in 55 min with a water-acetonitile gradient in the presence of trifluoroacetic acid. Detection was effected with a light-scattering detector. The detection limit was determined and was ca. 9 mu g of atractyloside standard injected. The method of quantification was validated.

引用

页码：119 / 122

页数：4

共 10 条

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