INDUCTION OF ADF/TRX BY OXIDATIVE STRESS IN KERATINOCYTES AND LYMPHOID-CELLS

被引：96

作者：

SACHI, Y

HIROTA, K

MASUTANI, H

TODA, K

TAKASHI, O

TAKIGAWA, M

YODOI, J

机构：

[1] KYOTO UNIV, INST VIRUS RES, DEPT BIOL RESPONSES, SAKYO KU, KYOTO 60601, JAPAN

[2] KYOTO UNIV, DEPT DERMATOL, SAKYO KU, KYOTO 60601, JAPAN

[3] HAMAMATSU UNIV SCH MED, DEPT DERMATOL, HAMAMATSU, SHIZUOKA 43131, JAPAN

[4] NAGOYA CITY UNIV, SCH MED, DEPT MOLEC GENET, MIZUHO KU, NAGOYA, AICHI 467, JAPAN

来源：

IMMUNOLOGY LETTERS | 1995年 / 44卷 / 2-3期

关键词：

THIOREDOXIN; ADULT T-CELL LEUKEMIA; ADULT T-CELL LEUKEMIA-DERIVED FACTOR; OXIDATIVE STRESS; ULTRAVIOLET LIGHT; KERATINOCYTE; HYDROGEN PEROXIDE;

D O I：

10.1016/0165-2478(95)00213-O

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Adult T-cell leukemia-derived factor (ADF)/human thioredoxin (TRX) has thiol-dependent reducing activities and is known to have regulatory roles on the DNA-protein interaction and cell activation. Inducive effect of ultraviolet (UV) has been indicated because of the enhanced expression of ADF/TRX in epidermal cells of sun-exposed skin, as determined by immunohistochemical staining with antibody against recombinant ADF (rADF). We studied the effect of UVB irradiation and other oxidative stress on the expression of ADF/TRX in epithelial cells as well as lymphoid cells, as HTLV-1 and Epstein-Barr virus-transformed lymphoid cells constitutively produce ADF/TRX. Using immunohistochemical staining anti-ADF antibody, the enhancement of ADF/TRX expression on primary culture of human keratinocytes was demonstrated, 12 h after 20 mJ/cm(2) UVB irradiation. Western blot analysis of the ADF/TRX protein in the cell lysates also showed the significant induction. In in situ hybridization, induction of ADF/TRX mRNA was detected after 4 h of UV exposure. ADF/TRX was also induced in a HTLV-1 (+) T-cell line, MT-1, by UVB or H2O2 dose dependently. The augmentation of ADF/TRX was observed 6 h after treatment of H2O2.

引用

页码：189 / 193

页数：5

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