ADSORPTION OF CELLULASES ON STEAM-PRETREATED WILLOW

被引：9

作者：

GALBE, M ^{[1
]}

EKLUND, R ^{[1
]}

ZACCHI, G ^{[1
]}

机构：

[1] UNIV LUND, POB 124, S-22100 LUND, SWEDEN

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1990年 / 24-5卷

关键词：

adsorption; cellulases; enzyme recovery; willow;

D O I：

10.1007/BF02920236

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The adsorption of cellulases on steam-pretreated willow has been measured for 2, 4, and 8 wt% willow and with varying enzyme concentrations (2-100 wt% based on substrate). The enzyme concentration was measured as soluble protein, filter paper activity (FPA), CMC-ase activity, and activity toward willow. The adsorption data were modeled with a Langmuir isotherm. The maximum adsorption capability depends on the method for measurement of the enzyme concentration. The lowest value, 470 mg enzyme/g willow, was obtained for the soluble protein and the highest value, 650 mg/g, for the FPA. For technical applications, a single isotherm can be used for calculation of the adsorption capability of steam-treated willow. © 1990 Humana Press Inc.

引用

页码：87 / 101

页数：15

共 22 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

GALBE M, 1986, BIOTECH BIOENG S, V17, P97

[3] MEASUREMENT OF CELLULASE ACTIVITIES [J].