PURIFICATION AND PARTIAL CHARACTERIZATION OF A FRUCTANASE WHICH HYDROLYZES NATURAL POLYSACCHARIDES FROM SUGARCANE JUICE

被引：27

作者：

LEGAZ, ME ^{[1
]}

MARTIN, L ^{[1
]}

PEDROSA, MM ^{[1
]}

VICENTE, C ^{[1
]}

DEARMAS, R ^{[1
]}

MARTINEZ, M ^{[1
]}

MEDINA, I ^{[1
]}

RODRIGUEZ, CW ^{[1
]}

机构：

[1] LA HAVANNE UNIV,FAC BIOL,DEPT PLANT PHYSIOL,HAVANNE,CUBA

来源：

PLANT PHYSIOLOGY | 1990年 / 92卷 / 03期

关键词：

D O I：

10.1104/pp.92.3.679

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A new sugarcane (Saccharum officinarum L.) fructanase which hydrolyzes both high molecular weight polysaccharides |Fructose4:Galactitol5|n (SP) and moderate-sized carbohydrates |Fructose2:Galactitol33|n (MMWC) has been purified from sugarcane juice. The Km value has been estimated to be 33.7 micrograms per milliliter and 20 micrograms per milliliter for SP and MMWC, respectively. The optimal pH and temperature values are 6.0 and 30°C, respectively. Purified protein has a pl value of 6.35 and a molecular weight of 13.2 kilodaltons. Fructanase activity appears to be Mn2+-dependent.

引用

页码：679 / 683

页数：5

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