IDENTIFICATION AND CHARACTERIZATION OF FOOT-AND-MOUTH-DISEASE VIRUS O1 BURGWEDEL/1987 AS AN INTERTYPIC RECOMBINANT

被引:35
作者
KREBS, O [1 ]
MARQUARDT, O [1 ]
机构
[1] FED RES CTR VIRUS DIS ANIM,POB 1149,W-7400 TUBINGEN,GERMANY
关键词
D O I
10.1099/0022-1317-73-3-613
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The foot-and-mouth disease virus field isolate Burgwedel/1987 subtype O1 was found to differ genetically from the antigenically related strain O1 Kaufbeuren within the region encoding the non-structural proteins. This genetic difference was indicated by the RNase mismatch cleavage method and confirmed by nucleotide sequencing. An alignment of sequences encoding proteinase 3C of the Burgwedel isolate and several other virus strains identified this isolate as an intertypic recombinant; the parent strains were O1 Kaufbeuren and a subtype C1 strain. Recombination occurred between nucleotide positions 5493 and 5221, within the region encoding peptide 3B1. Thus, the 5' three-quarters quarters of the O1 genome were fused to the 3'-terminal quarter of the C1 genome. Other contemporary isolates from the same district are not recombinants. Sequence alignment distinguished four patterns of proteinase 3C-coding sequences among the virus strains analysed: subtypes A12, C1 and O1 exhibit one pattern each, and another pattern is common to subtypes A5, A10 and O2.
引用
收藏
页码:613 / 619
页数:7
相关论文
共 28 条