CONJUGAL BLOCKS IN TETRAHYMENA PATTERN MUTANTS AND THEIR CYTOPLASMIC RESCUE .1. BROADENED-CORTICAL-DOMAINS (BCD)

被引：16

作者：

COLE, ES ^{[1
]}

机构：

[1] UNIV IOWA,DEPT BIOL,IOWA CITY,IA 52242

来源：

DEVELOPMENTAL BIOLOGY | 1991年 / 148卷 / 02期

关键词：

D O I：

10.1016/0012-1606(91)90260-A

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The cortical pattern mutant broadened cortical domains (bcd) in Tetrahymena thermophila is unable to complete the nuclear events associated with conjugation. bcd × bcd pairs become arrested at the "nuclear exchange" configuration. Genetic analysis reveals that the bcd conjugal block is 100% penetrant, under macronuclear control, and rescueable (a) by outcrossing to a wild-type partner, (b) by administration of a hyperosmotic shock 5 hr after cells are mixed for mating, or (c) by cytoplasmic transfusion from a wild-type donor. Cytological analysis reveals that the conjugal block is primarily the result of failure in pronuclear fusion (karyogamy). bcd pairs also exhibit reduced nuclear exchange efficiency and a failure of macronuclear anlagen formation. The hypothesis is proposed that the bcd+ gene codes for a microtubule-based organelle "motor" similar to kinesin. © 1991.

引用

页码：403 / 419

页数：17

共 38 条

[1] GENOMIC EXCLUSION - A RAPID MEANS FOR INDUCING HOMOZYGOUS DIPLOID LINES IN TETRAHYMENA PYRIFORMIS SYNGEN I [J].