IN-VIVO EPR MEASUREMENT OF RADICAL REACTION IN WHOLE MICE - INFLUENCE OF INSPIRED OXYGEN AND ISCHEMIA - REPERFUSION INJURY ON NITROXIDE REDUCTION

被引：32

作者：

UTSUMI, H

TAKESHITA, K

MIURA, Y

MASUDA, S

HAMADA, A

机构：

[1] Department of Health Chemistry, School of Pharmaceutical Sciences, Shown University, Tokyo, Hatanodai, Shinagawa

来源：

FREE RADICAL RESEARCH COMMUNICATIONS | 1993年 / 19卷

关键词：

L-BAND EPR; NITROXIDE RADICAL; ACTIVE OXYGENS; ISCHEMIA-REPERFUSION; OXYGEN; IN-VIVO;

D O I：

10.3109/10715769309056s219

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In vivo EPR measurements were carried out with whole mice to evaluate the influence of inspired oxygen and ischemia-reperfusion injury on spin-clearance of the nitroxide radicals which were administered intravenously or intramuscularly. Nitroxide radicals in head, abdomen, or muscle domains were composed of sharp triplet lines. The peak heights decreased gradually with time. The reduction of nitroxide radicals depended both on the inspired oxygen concentration and on the domains. Femoral ischemia-reperfusion injury also affected spin-clearance of the nitroxide radical in the thigh. The results were discussed with regard to the generation of active oxygen species.

引用

页码：S219 / S225

页数：7

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