SIMILARITIES BETWEEN BETA-AMYLOID PEPTIDE-1-40 AND PEPTIDE-40-1 - EFFECTS ON AGGREGATION, TOXICITY IN-VITRO, AND INJECTION IN YOUNG AND AGED RATS

被引:41
作者
GIORDANO, T
PAN, JB
MONTEGGIA, LM
HOLZMAN, TF
SNYDER, SW
KRAFFT, G
GHANBARI, H
KOWALL, NW
机构
[1] ABBOTT LABS,DEPT NEUROSCI,ABBOTT PK,IL 60064
[2] ABBOTT LABS,DEPT DRUG DESIGN & DELIVERY,ABBOTT PK,IL 60064
[3] ABBOTT LABS,DEPT NEUROPSYCHIAT MARKERS,ABBOTT PK,IL 60064
[4] VET ADM MED CTR,CTR GERIATR RES EDUC,BEDFORD,MA 01730
[5] BOSTON UNIV,DEPT NEUROL & PATHOL,BEDFORD,MA 01730
关键词
D O I
10.1006/exnr.1994.1022
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Peptides corresponding to the first 40 amino acids of beta amyloid peptide (beta 1-40) and the reverse sequence (beta 40-1) were synthesized, purified, and compared for their ability to aggregate and cause toxicity in vitro to human neuroblastoma cells (SH-SY5Y), as well as for effects following injection into young or aged rats. Aggregation of both peptides produced similar sedimentation velocity profiles and resulted in significant toxicity in vitro with no observable differences between beta 1-40 and beta 40-1. In addition, when injected into the cortex of young rats, beta 1-40 was more toxic than beta 40-1 although both resulted in significant lesions. However, in aged rats the two peptides resulted in lesions of similar size. Alz 50 staining and abnormal neurites were associated with both beta 1-40 and beta 40-1 lesions; however, no evidence of plaques or tangles was found in either age group. While both peptides were toxic in vitro, only beta 1- 40 elicited Alz 50 staining of SH-SY5Y cells. Electron microscopic examination of beta 1-40 and beta 40-1 aggregates showed that beta 1-40 formed fibrillar structures whereas beta 40-1 resulted in amorphous particles. Thus, although both peptides were toxic to cultured cells and aged rats, the toxicities may have resulted from different mechanisms. (C) 1994 Academic Press, Inc.
引用
收藏
页码:175 / 182
页数:8
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